| Place | Performance | Athlete | Team |
| 1 | 12.01 | Q: KOOPMAN - TAYGEN | WESTERN PROVINCE ATHLETICS |
| 2 | 12.08 | Q: VAN DER MERWE - DENE | ATHLETICS GAUTENG NORTH |
| 3 | 12.12 | Q: JORDAAN - JUAN | ATHLETICS VAAL TRIANGLE |
| 4 | 12.15 | Q: NDUBUEZE - OLUCHI ABELWE | CENTRAL GAUTENG ATHLETICS |
| 5 | 12.59 | q: OKOYE - PARIS | CENTRAL GAUTENG ATHLETICS |
| 6 | 12.78 | q: SMITH - SUZELLE | ATHLETICS CENTRAL NORTHWEST |
| 7 | 13.38 | -: JESILE - ASIPHESONA | EASTERN PROVINCE ATHLETICS |
| 8 | 13.87 | -: DU PREEZ - KAYLA | EASTERN PROVINCE ATHLETICS |