| Place | Performance | Athlete | Team |
| 1 | 12.17 | Q: KONDE - FAITH | BOLAND ATHLETICS |
| 2 | 12.55 | Q: FOURIE - KATE-LYNN | ATHLETICS CENTRAL NORTHWEST |
| 3 | 12.65 | Q: MDAKA - IMITHA | CENTRAL GAUTENG ATHLETICS |
| 4 | 12.81 | Q: NIEHAUS - CHAN HAYLEY | CENTRAL GAUTENG ATHLETICS |
| 5 | 12.99 | -: KLEYNHANS - IGNA | ATHLETICS SOUTHWESTERN DISTRICTS |
| 6 | 13.32 | -: NJAPHA - PHILASANDE | KWAZULU-NATAL ATHLETICS |
| 7 | 14.05 | -: STEYN - MEGAN | LIMPOPO ATHLETICS |