| Place | Performance | Athlete | Team |
| 1 | 24.34 | Q: LA GRANGE - KAYLA | UNIVERSITY OF JOHANNESBURG A |
| 2 | 24.79 | Q: VOSLOO - LEONETTE | UNIVERSITY OF PRETORIA A |
| 3 | 24.89 | -: COMPAAN - MILA | UNIVERSITY OF PRETORIA A |
| 4 | 25.01 | -: NGEMA - SNETHEMBA | UNIVERSITY OF KWAZULU-NATAL |
| 5 | 26.55 | -: DU PLESSIS - STEFANI | STELLENBOSCH UNIVERSITY |
| 6 | 27.86 | -: HANNAH - STEPHENS | UNIVERSITY OF WESTERN CAPE A |
| 7 | -: STIGLINGH - BERNICE | UNIVERSITY OF PRETORIA B | |
| 8 | -: DE JAGER - RENCIA | UNIVERSITY OF WESTERN CAPE A |