| Place | Performance | Athlete | Team |
| 1 | 24.45 | Q: MBISHA - JOVIALE | UNIVERSITY OF JOHANNESBURG A |
| 2 | 24.57 | Q: FOURIE - ANNAME | UNIVERSITY OF FREE STATE A |
| 3 | 24.99 | -: JOHNSON - LARA | STELLENBOSCH UNIVERSITY |
| 4 | 25.56 | -: OOSTHUIZEN - SYDNEY | NORTHWEST UNIV POTCHEFSTROOM B |
| 5 | 26.20 | -: SEBOGODI - BASETSANA | UNIVERSITY OF FREE STATE A |
| 6 | 26.98 | -: GERMISSHUYSEN - EMMARENTIA | UNIVERSITY OF JOHANNESBURG B |
| 7 | 27.86 | -: MOOLA - HANAH | UNIVERSITY OF CAPE TOWN A |