| Place | Performance | Athlete | Team |
| 1 | 24.20 | Q: SHABANGU - BANELE | TSHWANE UNIV OF TECHNOLOGY |
| 2 | 24.80 | Q: LE ROUX - KAYLEE | UNIVERSITY OF JOHANNESBURG A |
| 3 | 25.07 | -: NDLELA - MELODY | DURBAN UNIV OF TECH |
| 4 | 25.37 | -: KOEKEMOER - DAYLE | VARSITY COLLEGE |
| 5 | 25.64 | -: BOOYSEN - MIENKE | UNIVERSITY OF PRETORIA A |
| 6 | 27.56 | -: MAMELASIGIDI - KAMOGELO | VAAL UNIV OF TECHNOLOGY |
| 7 | 28.74 | -: LOUW - LENICE | UNIVERSITY OF WESTERN CAPE A |