| Place | Performance | Athlete | Team |
| 1 | 26.00 | Q: KYEMBO - RAISSA | UNIVERSITY OF JOHANNESBURG A |
| 2 | 26.21 | Q: GERMISSHUYSEN - EMMARENTIA | UNIVERSITY OF JOHANNESBURG B |
| 3 | 28.29 | Q: LOUW - LENICE | UNIVERSITY OF WESTERN CAPE A |
| 4 | 29.97 | -: KAMOGELO - SIBANDA | TSHWANE UNIV OF TECHNOLOGY |