| Place | Performance | Athlete | Team |
| 1 | 24.99 | Q: LE ROUX - KAYLEE | UNIVERSITY OF JOHANNESBURG A |
| 2 | 25.00 | Q: NDLELA - MELODY | DURBAN UNIV OF TECH |
| 3 | 25.36 | Q: NGEMA - SNETHEMBA | UNIVERSITY OF KWAZULU-NATAL |
| 4 | 26.31 | -: SEBOGODI - BASETSANA | UNIVERSITY OF FREE STATE A |
| 5 | 26.76 | -: DU PLESSIS - STEFANI | STELLENBOSCH UNIVERSITY |
| 6 | 29.07 | -: JORDAAN - SALMA | UNIVERSITY OF CAPE TOWN A |
| 7 | 30.26 | -: AMADI-AHANEKU - VANESSA | VAAL UNIV OF TECHNOLOGY |