| Place | Performance | Athlete | Team |
| 1 | 24.37 | Q: SHABANGU - BANELE | TSHWANE UNIV OF TECHNOLOGY |
| 2 | 25.59 | Q: OOSTHUIZEN - SYDNEY | NORTHWEST UNIV POTCHEFSTROOM B |
| 3 | 25.73 | Q: BOOYSEN - MIENKE | UNIVERSITY OF PRETORIA A |
| 4 | 25.75 | -: KOEKEMOER - DAYLE | VARSITY COLLEGE |
| 5 | 26.39 | -: STIGLINGH - BERNICE | UNIVERSITY OF PRETORIA B |
| 6 | 27.61 | -: MAMELASIGIDI - KAMOGELO | VAAL UNIV OF TECHNOLOGY |
| 7 | 29.86 | -: MANTSHA - MULWELI | VAAL UNIV OF TECHNOLOGY |