| Place | Performance | Athlete | Team |
| 1 | 25.08 | Q: JOHNSON - LARA | STELLENBOSCH UNIVERSITY |
| 2 | 25.26 | Q: VOSLOO - LEONETTE | UNIVERSITY OF PRETORIA A |
| 3 | 27.25 | Q: DE JAGER - RENCIA | UNIVERSITY OF WESTERN CAPE A |
| 4 | 27.30 | -: MAAKE - OFENTSE | TSHWANE UNIV OF TECHNOLOGY |
| 5 | 28.73 | -: DIBETLE - ORATILE | UNIVERSITY OF CAPE TOWN A |
| 6 | -: BANDA - FORTUNATE | UNIVERSITY OF MPUMALANGA |