| Place | Performance | Athlete | Team |
| 1 | 0:45.30 | Q: PILLAY - LYTHE | UNIVERSITY OF JOHANNESBURG A |
| 2 | 0:47.49 | Q: VAN DER WALT - TJAAT JOHAN | NELSON MANDELA UNIVERSITY |
| 3 | 0:48.77 | -: NYAMBI - MBUSO | UNIVERSITY OF JOHANNESBURG B |
| 4 | 0:49.11 | -: SNYDERS - JJ | UNIVERSITY OF FREE STATE A |
| 5 | 0:49.61 | -: MADONSELA - KARABO | UNIVERSITY OF PRETORIA B |
| 6 | 0:50.90 | -: MOKOENA - THAPELO | UNIVERSITY OF FREE STATE B |
| 7 | 0:51.31 | -: MBATHA - SIBONAKALISO | VAAL UNIV OF TECHNOLOGY |
| 8 | 0:52.55 | -: LANGA - MICLAS | TSHWANE UNIV OF TECHNOLOGY |