| Place | Performance | Athlete | Team |
| 1 | 0:46.76 | Q: MASEKO - NHLANHLA | UNIVERSITY OF JOHANNESBURG B |
| 2 | 0:47.51 | Q: MTHIMKULU - MTHI | UNIVERSITY OF FREE STATE A |
| 3 | 0:48.46 | -: KOTSEDI - MOTHEO | UNIVERSITY OF CAPE TOWN A |
| 4 | 0:49.26 | -: MBALULA - LONWABO | NELSON MANDELA UNIVERSITY |
| 5 | 0:49.63 | -: THOBIGUNYA - SIPHOSETHU | CAPE PENINSULA UNIV OF TECH |
| 6 | 0:51.97 | -: MANAMELA - KATLEGO | UNIVERSITY OF CAPE TOWN A |
| 7 | 0:52.21 | -: TEBBUTT - CODY | UNIVERSITY OF CAPE TOWN A |
| 8 | 0:52.98 | -: MOSAMEDI - MPHO | VAAL UNIV OF TECHNOLOGY |