| Place | Performance | Athlete | Team |
| 1 | 0:53.59 | Q: VILJOEN - MARLIE | UNIVERSITY OF PRETORIA A |
| 2 | 0:54.14 | Q: STRIJDOM - ANGELIQUE | TSHWANE UNIV OF TECHNOLOGY |
| 3 | 0:54.63 | Q: MOLEPO - PRECIOUS | TSHWANE UNIV OF TECHNOLOGY |
| 4 | 0:55.64 | q: DE WET - SIMONE | UNIVERSITY OF PRETORIA A |
| 5 | 0:55.97 | -: NAUDE - AMY | VARSITY COLLEGE |
| 6 | 1:08.50 | -: MASIZANA - YOLANDA | VAAL UNIV OF TECHNOLOGY |
| 7 | 1:10.88 | -: ELIAS - DENISCHA | UNIVERSITY OF WESTERN CAPE A |