| Place | Performance | Athlete | Team |
| 1 | 0:53.71 | Q: GIBBON - NICOLA | UNIVERSITY OF FREE STATE A |
| 2 | 0:54.92 | Q: VAN DER WALT - ZENEY | TSHWANE UNIV OF TECHNOLOGY |
| 3 | 0:55.20 | Q: MAGERMAN - GEZELLE | TSHWANE UNIV OF TECHNOLOGY |
| 4 | 0:55.33 | q: HATTINGH - CARLA | NORTHWEST UNIV POTCHEFSTROOM A |
| 5 | 0:55.69 | -: OOSTHUIZEN - MICHAELA | UNIVERSITY OF PRETORIA A |
| 6 | 0:56.29 | -: NEL - ANJE | UNIVERSITY OF PRETORIA A |
| 7 | 0:58.65 | -: BOOYSEN - MIENKE | UNIVERSITY OF PRETORIA A |