| Place | Performance | Athlete | Team |
| 1 | 0:48.65 | Q: KOTSEDI - MOTHEO | UNIVERSITY OF CAPE TOWN A |
| 2 | 0:48.90 | Q: SAMANGA - ANGELO | UNIVERSITY OF WESTERN CAPE A |
| 3 | 0:49.19 | Q: SNYDERS - JJ | UNIVERSITY OF FREE STATE A |
| 4 | 0:50.12 | Q: MADONSELA - KARABO | UNIVERSITY OF PRETORIA B |
| 5 | 0:50.14 | q: MALULEKA - LASSIE | VAAL UNIV OF TECHNOLOGY |
| 6 | 0:53.55 | -: NTSAKO - CHAUKE | VAAL UNIV OF TECHNOLOGY |