| Place | Performance | Athlete | Team |
| 1 | 0:47.61 | Q: MTHIMKULU - MTHI | UNIVERSITY OF FREE STATE A |
| 2 | 0:47.75 | Q: VAN DER WALT - TJAAT JOHAN | NELSON MANDELA UNIVERSITY |
| 3 | 0:48.28 | Q: STADLER - JOHAN | STELLENBOSCH UNIVERSITY |
| 4 | 0:50.55 | Q: MATLALA - THATO | UNIVERSITY OF PRETORIA A |
| 5 | 0:51.85 | q: MOSAMEDI - MPHO | VAAL UNIV OF TECHNOLOGY |
| 6 | 0:52.06 | -: DE VOS - MC DONALD | UNIVERSITY OF WESTERN CAPE A |
| 7 | 0:53.88 | -: MARKETOS - WILLIAM | UNIVERSITY OF CAPE TOWN A |