| Place | Performance | Athlete | Team |
| 1 | 0:48.67 | Q: MASEKO - NHLANHLA | UNIVERSITY OF JOHANNESBURG B |
| 2 | 0:49.81 | Q: MBALULA - LONWABO | NELSON MANDELA UNIVERSITY |
| 3 | 0:50.22 | Q: NEFDT - ROMANI | UNIVERSITY OF WESTERN CAPE B |
| 4 | 0:51.17 | Q: MBATHA - SIBONAKALISO | VAAL UNIV OF TECHNOLOGY |
| 5 | 0:52.49 | -: ADAMS - REHANE | UNIVERSITY OF WESTERN CAPE A |
| 6 | 0:52.75 | -: MABULA - NKOAI | UNIVERSITY OF FREE STATE B |
| 7 | 0:56.78 | -: PARKS - FIDEL | UNIVERSITY OF CAPE TOWN A |