| Place | Performance | Athlete | Team |
| 1 | 0:48.23 | Q: KHOZA - SIPHESIHLE | UNIVERSITY OF PRETORIA B |
| 2 | 0:48.40 | Q: NYAMBI - MBUSO | UNIVERSITY OF JOHANNESBURG B |
| 3 | 0:48.95 | Q: MOHOLOBELA - KABELO | UNIVERSITY OF JOHANNESBURG B |
| 4 | 0:49.94 | Q: THOBIGUNYA - SIPHOSETHU | CAPE PENINSULA UNIV OF TECH |
| 5 | 0:50.23 | q: JAMBAWO - MUNASHE | UNIVERSITY OF CAPE TOWN A |
| 6 | 0:50.42 | q: MOKOENA - THAPELO | UNIVERSITY OF FREE STATE B |
| 7 | 0:53.94 | -: NYASHANU - NIGEL | UNIVERSITY OF WESTERN CAPE A |