| Place | Performance | Athlete | Team |
| 1 | 0:48.72 | Q: PILLAY - LYTHE | UNIVERSITY OF JOHANNESBURG A |
| 2 | 0:51.67 | Q: TEBBUTT - CODY | UNIVERSITY OF CAPE TOWN A |
| 3 | 0:52.17 | Q: MANAMELA - KATLEGO | UNIVERSITY OF CAPE TOWN A |
| 4 | 0:52.28 | Q: LANGA - MICLAS | TSHWANE UNIV OF TECHNOLOGY |
| 5 | 0:56.90 | -: MOKOATSIE - TSHIDISANG | UNIVERSITY OF MPUMALANGA |
| 6 | 0:57.76 | -: GABADISE - HILTON | CENTRAL UNIV OF TECH BFN |