| Place | Performance | Athlete | Team |
| 1 | 43.01 | PIENAAR - DANYELLE | FREESTATE |
| 2 | 43.11 | BASSON - KYLIE | WESTERN CAPE |
| 3 | 43.47 | GUNTER - ISABELLA | GAUTENG |
| 4 | 44.13 | JENKINS - CAYLEN | GAUTENG |
| 5 | 44.72 | LAMPRECHT - IZAAN | EASTERN CAPE |
| 6 | 45.61 | TRUTER - MARI | FREESTATE |
| 7 | 45.78 | DE KOCK - MIENKA | FREESTATE |
| 8 | 48.13 | XABA - SIYANDA | MPUMALANGA |