| Place | Performance | Athlete | Team |
| 1 | 23.14 | Q: MNQABI - MZOKHONA | KWA-ZULU NATAL LSEN |
| 2 | 23.45 | q: APHANE - KABELO | GAUTENG LSEN |
| 3 | 23.78 | -: MOLOKO - TLOU | MPUMALANGA LSEN |
| 4 | 24.41 | -: TSHABALALA - SPHELELE | KWA-ZULU NATAL LSEN |
| 5 | 25.63 | -: TSEOLA - TSHEPISO | GAUTENG LSEN |