| Place | Performance | Athlete | Team |
| 1 | 27.66 | Q: DE GOUVEIA - KAYLA | WESTERN CAPE LSEN |
| 2 | 27.66 | Q: JORDAAN - NISHE | GAUTENG LSEN |
| 3 | 29.18 | Q: JUANITA - BUYS | FREESTATE LSEN |
| 4 | 30.40 | q: HULBERT - ELMARIE | WESTERN CAPE LSEN |
| 5 | 30.48 | q: MASHININI - PUSELETSO | MPUMALANGA LSEN |
| 6 | 31.90 | -: MHLONGO - TARVANA | KWA-ZULU NATAL LSEN |
| 7 | 33.87 | -: VAN ROOI - DENISHIA | NORTHERN CAPE LSEN |