| Place | Performance | Athlete | Team |
| 1 | 28.94 | Q: VILANKULU - AMKELANI | MPUMALANGA LSEN |
| 2 | 29.29 | Q: BIANCHINA - CHANTEL | FREESTATE LSEN |
| 3 | 29.39 | Q: MOKOTJO - NTHABISENG | GAUTENG LSEN |
| 4 | 30.91 | -: OLIPHANT - LIZANDRE | WESTERN CAPE LSEN |
| 5 | 32.09 | -: WILSCHUT - JAIME-LEE | WESTERN CAPE LSEN |
| 6 | 33.48 | -: NZIMANDE - APHIWE | KWA-ZULU NATAL LSEN |