| Place | Performance | Athlete | Team |
| 1 | 24.22 | Q: MAPONYA - TSHEKO I | LIMPOPO LSEN |
| 2 | 24.22 | Q: LYTJIES - MIKYLE | WESTERN CAPE LSEN |
| 3 | 24.80 | -: SMIT - JASON-LEE | GAUTENG LSEN |
| 4 | 24.91 | -: OLERATO - TLHABANG | FREESTATE LSEN |
| 5 | 26.34 | -: MNISI - KHAYELIHLE | MPUMALANGA LSEN |
| 6 | 26.49 | -: HLATSWAYO - BANELE | MPUMALANGA LSEN |