| Place | Performance | Athlete | Team |
| 1 | 23.89 | q: NKOSI - WANDILE | MPUMALANGA LSEN |
| 2 | 24.26 | q: VAN WYK - MARSHALLY | WESTERN CAPE LSEN |
| 3 | 25.05 | q: VISSER - SEBASTIAN | WESTERN CAPE LSEN |
| 4 | 25.30 | q: JUNIOR - RAMOKHOASE | FREESTATE LSEN |
| 5 | 25.31 | q: DUBE - SIYABONGA | GAUTENG LSEN |
| 6 | 25.53 | -: VASSEN - DELSHAAN | GAUTENG LSEN |
| 7 | 25.69 | -: NICO - VILJOEN | FREESTATE LSEN |
| 8 | 25.90 | -: JANNETJIES - NOMARIO | NORTHERN CAPE LSEN |