| Place | Performance | Athlete | Team |
| 1 | 24.30 | q: SEFIKA - RETHABILE | GAUTENG LSEN |
| 2 | 24.71 | q: MAGODLA - NILHIPE | WESTERN CAPE LSEN |
| 3 | 25.24 | q: BENJAMINE - DIYANI | WESTERN CAPE LSEN |
| 4 | 25.59 | -: GERALDO - PIETERSE | FREESTATE LSEN |
| 5 | 25.84 | -: DLAMINI - THANDA | MPUMALANGA LSEN |
| 6 | 26.04 | -: KONDILE - LUMI OYISA | EASTERN CAPE LSEN |
| 7 | 27.71 | -: DEORAJ - TYRAL | KWA-ZULU NATAL LSEN |