| Place | Performance | Athlete | Team |
| 1 | 29.07 | Q: DUBE - OWAMI | GAUTENG LSEN |
| 2 | 29.89 | Q: HESSELMAN - ANICKA | MPUMALANGA LSEN |
| 3 | 29.94 | Q: JONATHAN - KEYSHIA | GAUTENG LSEN |
| 4 | 30.17 | q: MADISHA - KATLEGO | GAUTENG LSEN |
| 5 | 30.36 | -: WALTERS - TAMIA | KWA-ZULU NATAL LSEN |
| 6 | 30.42 | -: BOOYSEN - ASHLIN | WESTERN CAPE LSEN |
| 7 | 31.31 | -: BOKANG - MPUTHI | FREESTATE LSEN |
| 8 | 31.94 | -: BLOEM - EVE | NORTHWEST LSEN |