| Place | Performance | Athlete | Team |
| 1 | 25.23 | Q: BURGER - RUM | FREESTATE |
| 2 | 25.47 | Q: CHISOM - UDENZE | WESTERN CAPE |
| 3 | 25.60 | q: GUNTER - ISABELLA | GAUTENG |
| 4 | 25.78 | -: BASSON - KYLIE | WESTERN CAPE |
| 5 | 26.99 | -: TIVANE - SONIA | MPUMALANGA |
| 6 | 28.25 | -: NOVELA - MONICA | LIMPOPO |