| Place | Performance | Athlete | Team |
| 1 | 0:53.91 | Q: NTIYISO - NKANYANI | MPUMALANGA |
| 2 | 0:54.20 | Q: NAUDE - ZANDER | GAUTENG |
| 3 | 0:54.59 | Q: BROODRYK - GERT | FREESTATE |
| 4 | 0:54.66 | q: WESSELS - WESSEL | FREESTATE |
| 5 | 0:55.90 | q: MAKOTSO - KAMAHELO | FREESTATE |
| 6 | 0:58.96 | -: STADLER - ESTEFAN | WESTERN CAPE |