| Place | Performance | Athlete | Team |
| 1 | 0:53.49 | Q: BERNING - TYRONE | MPUMALANGA |
| 2 | 0:54.27 | Q: MARITZ - DELANO | GAUTENG |
| 3 | 0:55.75 | Q: RIO - MAURICE | WESTERN CAPE |
| 4 | 0:56.38 | -: MAJAKE - KATLEGO | MPUMALANGA |
| 5 | 0:56.40 | -: VAN HEERDEN - HENDRICO | NORTHWEST PROVINCE |
| 6 | 0:59.79 | -: TOLI - BOAGO | NORTHERN CAPE |