| Place | Performance | Athlete | Team |
| 1 | 40.32 | Q: MYBURG - STIAAN | GAUTENG |
| 2 | 40.73 | Q: BALYOI - KATLEGO | MPUMALANGA |
| 3 | 40.94 | Q: MEIRING - SIMON | WESTERN CAPE |
| 4 | 40.96 | q: TIETUS - KEELAN | NORTHWEST PROVINCE |
| 5 | 41.44 | q: NIEUWOUDT - EVAN | FREESTATE |
| 6 | 42.36 | -: HUMAN - HERMANUS JOHANNES | NORTHWEST PROVINCE |
| 7 | 43.45 | -: BURGER - JAN-RE | MPUMALANGA |