| Place | Performance | Athlete | Team |
| 1 | 40.02 | Q: JAFTHA - RAQIEL | FREESTATE |
| 2 | 40.33 | Q: VAN DER WESTHUIZEN - JORDAN | GAUTENG |
| 3 | 40.93 | Q: RASOOL - MOHAMMED | FREESTATE |
| 4 | 41.98 | -: BROOKS - LEAGON | WESTERN CAPE |
| 5 | 42.03 | -: REDGARD - BRANDON | GAUTENG |
| 6 | 42.42 | -: HAASBROEK - JOHAN | LIMPOPO |
| 7 | 43.05 | -: MKHUMANE - LIZWE | MPUMALANGA |