| Place | Performance | Athlete | Team |
| 1 | 45.58 | Q: TRUTER - MARI | FREESTATE |
| 2 | 46.53 | Q: XABA - SIYANDA | MPUMALANGA |
| 3 | 46.59 | Q: JENKINS - CAYLEN | GAUTENG |
| 4 | 46.76 | -: VAN DER WALT - MIEKE | MPUMALANGA |
| 5 | 47.17 | -: JULIUS - ABBIE | KWA-ZULU NATAL |
| 6 | 47.21 | -: BOTES - KAILEY | LIMPOPO |
| 7 | 47.24 | -: UYS - EMMA | WESTERN CAPE |
| 8 | 55.17 | -: ERASMUS - ERNINE | NORTHERN CAPE |