| Place | Performance | Athlete | Team |
| 1 | 43.69 | Q: PIENAAR - DANYELLE | FREESTATE |
| 2 | 44.37 | Q: BASSON - KYLIE | WESTERN CAPE |
| 3 | 44.99 | Q: GUNTER - ISABELLA | GAUTENG |
| 4 | 45.47 | q: LAMPRECHT - IZAAN | EASTERN CAPE |
| 5 | 46.39 | q: DE KOCK - MIENKA | FREESTATE |
| 6 | 47.34 | -: VAN TONDER - MIEKE | EASTERN CAPE |
| 7 | 47.69 | -: HAMILTON - MEGAN | GAUTENG |
| 8 | 48.45 | -: BOSHOFF - VIDA | KWA-ZULU NATAL |