| Place | Performance | Athlete | Team |
| 1 | 24.99 | Q: VERMEULEN - CHANE | GAUTENG |
| 2 | 25.27 | Q: SMIDT - CHARDONE | GAUTENG |
| 3 | 25.80 | q: MIENIE - MIEKIE | LIMPOPO |
| 4 | 25.95 | q: VAN NIEKERK - TENINKE | MPUMALANGA |
| 5 | 26.16 | -: KIRSTEN - BRENDA | MPUMALANGA |
| 6 | 26.27 | -: RAMPALANG - JUSTINE | FREESTATE |
| 7 | 26.35 | -: KOTZ - AMY | FREESTATE |