| Place | Performance | Athlete | Team |
| 1 | 25.09 | Q: VAN OUDTSHOORN - KAYLIE | GAUTENG |
| 2 | 25.77 | Q: EHLERS - LARA | WESTERN CAPE |
| 3 | 26.05 | -: DE BEER - MIA | NORTHWEST PROVINCE |
| 4 | 26.23 | -: MEHLAPE - RELEBOGILE | LIMPOPO |
| 5 | 26.44 | -: VAN NIEKERK - AMELIE | NORTHERN CAPE |
| 6 | 27.22 | -: KHOMO - MPHO GLADYS | FREESTATE |
| 7 | 27.98 | -: MARAIS - ERYNNE | KWA-ZULU NATAL |