| Place | Performance | Athlete | Team |
| 1 | 23.01 | Q: COETZEE - JAEGER | WESTERN CAPE |
| 2 | 23.26 | Q: NDAWE - MATIMBA | MPUMALANGA |
| 3 | 23.35 | q: LAGARDIEN - AASIF | EASTERN CAPE |
| 4 | 23.38 | -: ABRAHAMS - NEO | KWA-ZULU NATAL |
| 5 | 24.07 | -: BADLI - KUNGAWO | EASTERN CAPE |
| 6 | 24.34 | -: VALENTINE - MATTHEW | FREESTATE |