| Place | Performance | Athlete | Team |
| 1 | 1:08.42 | Q: NXUMALO - LINDOKUHLE | GAUTENG LSEN |
| 2 | 1:09.50 | Q: MBETHE - ASEZILE | EASTERN CAPE LSEN |
| 3 | 1:11.55 | Q: OLIPHANT - LIZANDRE | WESTERN CAPE LSEN |
| 4 | 1:20.12 | -: SARALINA - JOLENE | WESTERN CAPE LSEN |
| 5 | 1:23.27 | -: REFILOE - PHAKIRA | FREESTATE LSEN |