| Place | Performance | Athlete | Team |
| 1 | 0:50.90 | Q: SINDANE - SIPHO | MPUMALANGA LSEN |
| 2 | 0:51.73 | Q: OLIPHANT - HAYGAN | GAUTENG LSEN |
| 3 | 0:52.31 | -: KAZADI - KABONGO | GAUTENG LSEN |
| 4 | 0:53.62 | -: SIWELA - NEO | GAUTENG LSEN |
| 5 | 0:54.72 | -: HARTS - PERSTIN | WESTERN CAPE LSEN |
| 6 | 0:55.29 | -: JACOBS (D) - JUSTINE | GAUTENG LSEN |
| 7 | 0:56.23 | -: COKOTO - HLUMANI | KWA-ZULU NATAL LSEN |