| Place | Performance | Athlete | Team |
| 1 | 0:56.21 | Q: BENJAMINE - DIYANI | WESTERN CAPE LSEN |
| 2 | 0:57.23 | Q: SEFIKA - RETHABILE | GAUTENG LSEN |
| 3 | 0:57.41 | Q: TITUS - JAY-T | WESTERN CAPE LSEN |
| 4 | 1:00.50 | -: SIBULAWA - FRANCE IMI | EASTERN CAPE LSEN |
| 5 | 1:02.94 | -: FREEMAN - DEVANDRE | NORTHWEST LSEN |
| 6 | 1:04.11 | -: SAMBO - AZILE | MPUMALANGA LSEN |