| Place | Performance | Athlete | Team |
| 1 | 0:49.20 | Q: VAN DER WATH - HEINRICH | FREESTATE |
| 2 | 0:50.12 | Q: RAPHULU - EMMANUEL | LIMPOPO |
| 3 | 0:50.54 | -: SINGWANE - PHILLIP | FREESTATE |
| 4 | 0:51.05 | -: MAKOTSO - KAMAHELO | FREESTATE |
| 5 | 0:52.14 | -: ELISHA - THAPELO | NORTHWEST PROVINCE |
| 6 | 0:54.09 | -: GOVENDER - VEESHAN | KWA-ZULU NATAL |