| Place | Performance | Athlete | Team |
| 1 | 0:48.96 | Q: GELDENHUYS - WIHAN | MPUMALANGA |
| 2 | 0:49.46 | Q: NCUBE - JAMES | GAUTENG |
| 3 | 0:50.18 | -: MOKHABELE - KHAMOHELO | FREESTATE |
| 4 | 0:50.50 | -: LOMBAARDT - DUWAYNE | WESTERN CAPE |
| 5 | 0:50.53 | -: DHLAKAMA - TASHUNGA | LIMPOPO |
| 6 | 0:50.62 | -: THOMAS - HILTON | GAUTENG |
| 7 | 0:51.57 | -: VILAKAZI - MFANAFUTHI | KWA-ZULU NATAL |