| Place | Performance | Athlete | Team |
| 1 | 0:57.08 | Q: VERMEULEN - CHANE | GAUTENG |
| 2 | 0:57.29 | Q: ESTERHUYSE - VENE | GAUTENG |
| 3 | 0:57.96 | Q: KINNEAR - HAYLEIGH | GAUTENG |
| 4 | 0:58.01 | -: EHLERS - LARA | WESTERN CAPE |
| 5 | 1:00.54 | -: REINSTEIN - NOA | KWA-ZULU NATAL |
| 6 | 1:02.88 | -: PHOGOLE - KOPANO | MPUMALANGA |
| 7 | 1:04.40 | -: BREYTENBACH - KAYLEIGH | KWA-ZULU NATAL |