| Place | Performance | Athlete | Team |
| 1 | 0:58.87 | Q: BINNEMAN - ENYA | WESTERN CAPE |
| 2 | 0:59.10 | Q: DE VILLIERS - JOHANKA | GAUTENG |
| 3 | 0:59.23 | Q: FOURIE - ASTRID | GAUTENG |
| 4 | 1:01.81 | -: REYNOLDS - ELNE | EASTERN CAPE |
| 5 | 1:02.06 | -: DE KOCK - MIENKA | FREESTATE |
| 6 | 1:02.47 | -: FICK - ROXY-LEE | MPUMALANGA |
| 7 | 1:07.97 | -: PETERS - TATUM | KWA-ZULU NATAL |