| Place | Performance | Athlete | Team |
| 1 | 27.37 | Q: DE BRUIN - HENRE | GAUTENG |
| 2 | 27.39 | Q: JAMIE - DONCASTER | MPUMALANGA |
| 3 | 28.67 | q: KGOSIENG - LETLHOGONOLO | NORTHWEST PROVINCE |
| 4 | 29.62 | -: AMBROSE - SHANE | WESTERN CAPE |
| 5 | 29.71 | -: MUDILA - CURTIS | GAUTENG |
| 6 | 30.03 | -: MANZINI - HUMBLE | LIMPOPO |
| 7 | 31.56 | -: KENT - TYLER | WESTERN CAPE |