| Place | Performance | Athlete | Team |
| 1 | 30.48 | Q: VORSTER - TAYLA | GAUTENG |
| 2 | 30.48 | Q: DU PREEZ - DIANE | NORTHWEST PROVINCE |
| 3 | 30.92 | q: BOTHA - MIENKE | FREESTATE |
| 4 | 31.47 | -: GEORGE - BRIANA | WESTERN CAPE |
| 5 | 32.48 | -: MATHEWE - THULISWA | FREESTATE |
| 6 | 34.67 | -: GWASARA - NAMARA | LIMPOPO |