| Place | Performance | Athlete | Team |
| 1 | 22.97 | Q: BOOYSEN - ELIZABETH | WESTERN CAPE |
| 2 | 23.32 | Q: JANSEN - ILKE | MPUMALANGA |
| 3 | 24.03 | -: PRINSLOO - ABBI-GAYLE | GAUTENG |
| 4 | 24.60 | -: DE KOCK - ANJE | FREESTATE |
| 5 | 26.54 | -: BYLSMA - Dunay | NORTHERN CAPE |
| 6 | -: KGANYAGO - KHOLOFELO | LIMPOPO |