| Place | Performance | Athlete | Team |
| 1 | 22.31 | Q: DU TOIT - LENKA | GAUTENG |
| 2 | 22.35 | Q: ROUX - IMKE | WESTERN CAPE |
| 3 | 22.90 | q: DU PLESSIS - ANNEMIE | WESTERN CAPE |
| 4 | 24.32 | -: POTGIETER - RIAN | LIMPOPO |
| 5 | 26.81 | -: SHINYANGANI - AMEND | LIMPOPO |
| 6 | 26.83 | -: DE JAGER - SANMARI | NORTHWEST PROVINCE |
| 7 | 64.93 | -: MOLOI - MICHELLE | FREESTATE |