| Place | Performance | Athlete | Team |
| 1 | 22.64 | Q: VOSLOO - LIENKE | EASTERN CAPE |
| 2 | 22.99 | Q: BELLINGHAM - HAZEL-MARI | MPUMALANGA |
| 3 | 23.50 | q: LUES - HAYLEY | NORTHWEST PROVINCE |
| 4 | 24.14 | -: MC LEOD - ISABEL | GAUTENG |
| 5 | 24.18 | -: HIPKIN - ALMA | MPUMALANGA |
| 6 | 25.28 | -: DE BOER - LIA | FREESTATE |
| 7 | 25.54 | -: BURGER - LINE | FREESTATE |