| Place | Performance | Athlete | Team |
| 1 | 04:56.65 | Q: MEYER - LEIGH-ANNE | FREESTATE |
| 2 | 04:57.01 | Q: MTHETHWA - AMAHLE | KWAZULU-NATAL |
| 3 | 05:00.69 | Q: PRINSLOO - SUNE-LOUISE | GAUTENG |
| 4 | 05:03.36 | Q: PRETORIUS - LEANDRI | GAUTENG |
| 5 | 05:03.68 | Q: MARHITHI - SINAWO | EASTERN CAPE |
| 6 | 05:07.70 | q: MAHLANGU - THANDOLWETHU | MPUMALANGA |
| 7 | 05:09.14 | q: THOMATHOS - TATIANA | WESTERN CAPE |
| 8 | 05:14.59 | q: NTSUMPA - ANGANATHI | EASTERN CAPE |
| 9 | 05:26.49 | -: GILIVITHI - ASANELE | EASTERN CAPE |
| 10 | 05:41.67 | -: TSOTETSI - AGNES | MPUMALANGA |
| 11 | 05:53.69 | -: MOHOLO - KELEBOGILE | NORTHWEST PROVINCE |