| Place | Performance | Athlete | Team |
| 1 | 4:04.71 | Q: ESTERHUIZEN - ZANE | WESTERN CAPE |
| 2 | 4:12.90 | Q: SEMAUSHU - KEAMOGETSWE | NORTHWEST PROVINCE |
| 3 | 4:13.26 | Q: MBHELE - SINOTHANDO | KWAZULU-NATAL |
| 4 | 4:15.40 | Q: DU PLESSIS - ANDREA | MPUMALANGA |
| 5 | 4:16.67 | Q: SYNMANN - MARNY | LIMPOPO |
| 6 | 4:17.89 | q: MAZONDO - BOKAMOSO | FREESTATE |
| 7 | 4:20.61 | q: NGOBELA - LISAKHANYA | EASTERN CAPE |
| 8 | 4:22.48 | q: VENTER - KLARA | NORTHWEST PROVINCE |
| 9 | 5:02.76 | -: DLAMINI - THEMBELIHLE | KWAZULU-NATAL |